8TFD | pdb_00008tfd

Crystal structure of a stem-loop DNA aptamer complexed with SARS-CoV-2 nucleocapsid protein RNA-binding domain

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	SARS coronavirus nucleoprotein RNA-binding domain-like	8092433	3000498	SCOP2B (2022-06-29)

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Nucleoprotein	molecular condensate scaffold activity protein-macromolecule adaptor activity molecular adaptor activity molecular sequestering activity protein sequestering activity RNA binding binding nucleic acid binding identical protein binding protein binding protein homodimerization activity protein dimerization activity RNA stem-loop binding MHC class I protein binding molecular condensate scaffold activity protein-macromolecule adaptor activity molecular adaptor activity molecular sequestering activity protein sequestering activity RNA binding binding nucleic acid binding identical protein binding protein binding protein homodimerization activity protein dimerization activity RNA stem-loop binding MHC class I protein binding signaling receptor binding MHC protein binding poly-pyrimidine tract binding poly(U) RNA binding single-stranded RNA binding	biological process involved in interspecies interaction between organisms response to stimulus response to other organism symbiont-mediated response to host defenses response to external stimulus response to biotic stimulus biological process involved in interaction with host biological process involved in symbiotic interaction response to host immune response response to host response to external biotic stimulus multicellular organismal process regulation of cellular process negative regulation of biological process biological process involved in interspecies interaction between organisms response to stimulus response to other organism symbiont-mediated response to host defenses response to external stimulus response to biotic stimulus biological process involved in interaction with host biological process involved in symbiotic interaction response to host immune response response to host response to external biotic stimulus multicellular organismal process regulation of cellular process negative regulation of biological process negative regulation of type I interferon production cytokine production negative regulation of cellular process regulation of cytokine production regulation of multicellular organismal process regulation of interferon-beta production interferon-beta production negative regulation of macromolecule metabolic process regulation of biosynthetic process regulation of biological process type I interferon production negative regulation of metabolic process biological regulation negative regulation of cytokine production regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of gene expression negative regulation of gene expression biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process negative regulation of interferon-beta production metabolic process cellular process regulation of macromolecule biosynthetic process macromolecule metabolic process negative regulation of multicellular organismal process regulation of metabolic process regulation of type I interferon production cellular response to stimulus positive regulation of cell communication positive regulation of response to biotic stimulus positive regulation of signal transduction pattern recognition receptor signaling pathway regulation of NLRP3 inflammasome complex assembly positive regulation of NLRP3 inflammasome complex assembly regulation of signal transduction intracellular signal transduction cell communication cellular component biogenesis immune response-activating signaling pathway cellular component assembly inflammasome-mediated signaling pathway positive regulation of immune response canonical inflammasome complex assembly regulation of immune system process signal transduction positive regulation of immune system process innate immune response-activating signaling pathway positive regulation of pattern recognition receptor signaling pathway immune response regulation of innate immune response positive regulation of intracellular signal transduction positive regulation of inflammasome-mediated signaling pathway regulation of inflammasome-mediated signaling pathway regulation of response to stimulus defense response to other organism regulation of pattern recognition receptor signaling pathway NLRP3 inflammasome complex assembly positive regulation of defense response activation of innate immune response regulation of immune response inflammatory response activation of immune response cytoplasmic pattern recognition receptor signaling pathway regulation of defense response regulation of response to biotic stimulus regulation of cellular component organization positive regulation of cellular component organization regulation of response to external stimulus intracellular receptor signaling pathway signaling positive regulation of biological process regulation of cytoplasmic pattern recognition receptor signaling pathway positive regulation of response to stimulus cellular component organization regulation of protein-containing complex assembly positive regulation of protein-containing complex assembly positive regulation of response to external stimulus immune response-regulating signaling pathway regulation of intracellular signal transduction regulation of response to stress response to stress innate immune response protein-containing complex assembly cellular component organization or biogenesis positive regulation of cellular process regulation of signaling positive regulation of innate immune response defense response protein-containing complex organization positive regulation of cellular component biogenesis defense response to symbiont regulation of cellular component biogenesis immune system process regulation of cell communication positive regulation of signaling virion assembly viral RNA genome packaging viral process viral life cycle viral genome packaging	host intracellular membrane-bounded organelle host cellular component host intracellular part other organism part host cell cytoplasm part host intracellular organelle host cell endoplasmic reticulum-Golgi intermediate compartment cellular anatomical structure host cell cytoplasm host cell part host intracellular region host extracellular space viral capsid virion component host intracellular membrane-bounded organelle host cellular component host intracellular part other organism part host cell cytoplasm part host intracellular organelle host cell endoplasmic reticulum-Golgi intermediate compartment cellular anatomical structure host cell cytoplasm host cell part host intracellular region host extracellular space viral capsid virion component MHC class I protein complex MHC protein complex cell periphery protein-containing complex plasma membrane protein complex membrane plasma membrane membrane protein complex intracellular anatomical structure cytoplasm host cell perinuclear region of cytoplasm intracellular organelle organelle membraneless organelle intracellular membraneless organelle extracellular region host cell Golgi apparatus ribonucleoprotein complex viral nucleocapsid
B	DNA aptamer	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR044344	Nucleocapsid (N) protein, C-terminal domain, coronavirus	Domain
A	IPR043505	Nucleocapsid protein, betacoronavirus	Family
A	IPR001218	Nucleocapsid protein, coronavirus	Family
A	IPR037195	Nucleocapsid protein, N-terminal	Homologous Superfamily
A	IPR037179	Nucleocapsid protein, C-terminal	Homologous Superfamily
A	IPR044345	Nucleocapsid (N) protein, N-terminal domain, coronavirus	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.