ELECTRON MICROSCOPY
| Sample |
|---|
| COMPLEX OF F-ACTIN, MYOE AND TROPOMYOSIN |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | FILAMENT |
| Vitrification Instrument | GATAN CRYOPLUNGE 3 |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | LIQUID ETHANE |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | HELICAL |
| Number of Particles | 5481 |
| Reported Resolution (Å) | 7.7 |
| Resolution Method | |
| Other Details | SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-1987 DEPOSITION ID: 10340). |
| Refinement Type | |
| Symmetry Type | HELICAL |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 (3MFP, 1LKX) | ||||
| Refinement Space | REAL | ||||
| Refinement Protocol | FLEXIBLE FIT | ||||
| Refinement Target | |||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | METHOD--FLEXIBLE FITTING BY DEN REFINEMENT PROTOCOL--EM | ||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | TVIPS TEMCAM-F816 (8k x 8k) | ||||||||
| Electron Dose (electrons/Å**2) | 1.7 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | |
| Temperature (Kelvin) | 77 |
| Microscope Model | JEOL 3200FSC |
| Minimum Defocus (nm) | 750 |
| Maximum Defocus (nm) | 1500 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | 4.1 |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | |
| Nominal Magnification | 80000 |
| Calibrated Magnification | 169644 |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 200 |
| Imaging Details | BEST 836 MICROGRAPHS WERE SELECTED FROM OVER 3000 AQUIRED IMAGES |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| MODEL FITTING | DireX | |
| RECONSTRUCTION | SPARX | |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| INDIVIDUAL PARTICLES | ||||
