Download MendeleyDNA Methyltransferase R165N Mutant
Daujotyte, D., Leinartaite, L., Grazulis, S.To be published.
2UZ4 | pdb_00002uz4
HhaI DNA methyltransferase R165N mutant complex with 13mer GCGC-GMGC oligonucleotide and SAH
- PDB DOI: https://doi.org/10.2210/pdb2UZ4/pdb
- NAKB: 2UZ4
- Classification: TRANSFERASE
- Organism(s): Haemophilus parahaemolyticus
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2007-04-25 Released: 2008-06-03
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.10 Å
- R-Value Free: 0.235 (Depositor), 0.220 (DCC)
- R-Value Work: 0.210 (Depositor), 0.200 (DCC)
- R-Value Observed: 0.210 (Depositor)
Starting Model: experimental
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This is version 1.4 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| MODIFICATION METHYLASE HHAI | 327 | Haemophilus parahaemolyticus | Mutation(s): 1 EC: 2.1.1.37 |  | |
UniProt | |||||
Find proteins for P05102 (Haemophilus parahaemolyticus) Explore P05102 Go to UniProtKB: P05102 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | P05102 | ||||
Sequence AnnotationsExpand | |||||
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Find similar nucleic acids by: Sequence | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | Organism | Image | |
| 5'-D(*TP*GP*GP*AP*TP*GP*5CMP*GP*CP*TP*GP *AP*C)-3' | B [auth C] | 13 | N/A |  | |
Sequence AnnotationsExpand | |||||
| |||||
Find similar nucleic acids by: Sequence | 3D Structure
Entity ID: 3 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Length | Organism | Image | |
| 5'-D(*GP*TP*CP*AP*GP*CP*GP*CP*AP*TP *CP*C)-3' | C [auth D] | 12 | N/A |  | |
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SAH Query on SAH | D [auth A] | S-ADENOSYL-L-HOMOCYSTEINE C14 H20 N6 O5 S ZJUKTBDSGOFHSH-WFMPWKQPSA-N |  | ||
| SO4 Query on SO4 | E [auth A], F [auth A], G [auth A], H [auth A] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
| GOL Query on GOL | I [auth A], J [auth A] | GLYCEROL C3 H8 O3 PEDCQBHIVMGVHV-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.10 Å
- R-Value Free: 0.235 (Depositor), 0.220 (DCC)
- R-Value Work: 0.210 (Depositor), 0.200 (DCC)
- R-Value Observed: 0.210 (Depositor)
Space Group: H 3 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 95.177 | α = 90 |
| b = 95.177 | β = 90 |
| c = 312.355 | γ = 120 |
| Software Name | Purpose |
|---|---|
| CNS | refinement |
| MOSFLM | data reduction |
| SCALA | data scaling |
| AMoRE | phasing |
Entry History
Deposition Data
- Released Date: 2008-06-03 Deposition Author(s): Daujotyte, D., Leinartaite, L., Grazulis, S.
Revision History (Full details and data files)
- Version 1.0: 2008-06-03
Type: Initial release - Version 1.1: 2011-05-08
Changes: Version format compliance - Version 1.2: 2011-07-13
Changes: Version format compliance - Version 1.3: 2019-07-24
Changes: Data collection, Derived calculations - Version 1.4: 2023-12-13
Changes: Data collection, Database references, Other, Refinement description
