Download MendeleyCatalytic bases in class II terpene cyclases
Pan, X.M., Dong, S.M., Dong, L.B.To be published.
8ZGW | pdb_00008zgw
Drimenyl diphosphate synthase SsDMS_F248Y&D303E from Streptomyces showdoensis in complex with farnesyl diphosphate (FPP) and Mg2+
- PDB DOI: https://doi.org/10.2210/pdb8ZGW/pdb
- Classification: BIOSYNTHETIC PROTEIN
- Organism(s): Streptomyces showdoensis
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2024-05-09 Released: 2025-05-21
- Funding Organization(s): National Natural Science Foundation of China (NSFC)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.23 Å
- R-Value Free: 0.245 (Depositor), 0.245 (DCC)
- R-Value Work: 0.189 (Depositor), 0.188 (DCC)
- R-Value Observed: 0.193 (Depositor)
Starting Model: experimental
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This is version 1.0 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Squalene cyclase C-terminal domain-containing protein | 539 | Streptomyces showdoensis | Mutation(s): 2 Gene Names: VO63_21045 EC: 5.4.99 |  | |
UniProt | |||||
Find proteins for A0A2P2GK84 (Streptomyces showdoensis) Explore A0A2P2GK84 Go to UniProtKB: A0A2P2GK84 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | A0A2P2GK84 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| FPP (Subject of Investigation/LOI) Query on FPP | B [auth A] | FARNESYL DIPHOSPHATE C15 H28 O7 P2 VWFJDQUYCIWHTN-YFVJMOTDSA-N |  | ||
| MG (Subject of Investigation/LOI) Query on MG | C [auth A], D [auth A] | MAGNESIUM ION Mg JLVVSXFLKOJNIY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.23 Å
- R-Value Free: 0.245 (Depositor), 0.245 (DCC)
- R-Value Work: 0.189 (Depositor), 0.188 (DCC)
- R-Value Observed: 0.193 (Depositor)
Space Group: C 2 2 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 51.32 | α = 90 |
| b = 113.88 | β = 90 |
| c = 180.089 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| iMOSFLM | data reduction |
| Aimless | data scaling |
| PHASER | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2025-05-21 Deposition Author(s): Pan, X.M., Dong, S.M., Dong, L.B.
| Funding Organization | Location | Grant Number |
|---|---|---|
| National Natural Science Foundation of China (NSFC) | China | 82073746 |
Revision History (Full details and data files)
- Version 1.0: 2025-05-21
Type: Initial release
