9KJZ | pdb_00009kjz

Cryo-EM structure of the Retron-Eco7 complex (state 3)


Experimental Data Snapshot

  • Method: ELECTRON MICROSCOPY
  • Resolution: 2.70 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 

wwPDB Validation   3D Report Full Report


This is version 1.0 of the entry. See complete history


Literature

Cryo-EM structure of the Retron-Eco7 complex

Ishikawa, J.Yoneyama, K.

To be published.

Macromolecules

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Entity ID: 1
MoleculeChains Sequence LengthOrganismDetailsImage
RNA-directed DNA polymerase322Escherichia coliMutation(s): 0 
Gene Names: D4M65_23810KQO22_004355
EC: 2.7.7.49
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Sequence Annotations
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  • Reference Sequence
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Entity ID: 2
MoleculeChains Sequence LengthOrganismDetailsImage
AAA family ATPase
B, C, D, E
550Escherichia coliMutation(s): 0 
Gene Names: OGW11_10610
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Entity ID: 3
MoleculeChains Sequence LengthOrganismDetailsImage
TIGR02646 family protein
F, G
266Escherichia coliMutation(s): 0 
Gene Names: D4M65_23820KQO22_004357
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  • Reference Sequence
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Entity ID: 4
MoleculeChains LengthOrganismImage
msrRNA146Escherichia coli
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Entity ID: 5
MoleculeChains LengthOrganismImage
msdDNA77Escherichia coli
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  • Reference Sequence
Small Molecules
Ligands 3 Unique
IDChains Name / Formula / InChI Key2D Diagram3D Interactions
ADP (Subject of Investigation/LOI)
Query on ADP

Download Ideal Coordinates CCD File 
J [auth B],
K [auth C],
L [auth E]
ADENOSINE-5'-DIPHOSPHATE
C10 H15 N5 O10 P2
XTWYTFMLZFPYCI-KQYNXXCUSA-N
ZN (Subject of Investigation/LOI)
Query on ZN

Download Ideal Coordinates CCD File 
M [auth F],
N [auth G]
ZINC ION
Zn
PTFCDOFLOPIGGS-UHFFFAOYSA-N
MG (Subject of Investigation/LOI)
Query on MG

Download Ideal Coordinates CCD File 
O [auth H]MAGNESIUM ION
Mg
JLVVSXFLKOJNIY-UHFFFAOYSA-N
Experimental Data & Validation

Experimental Data

  • Method: ELECTRON MICROSCOPY
  • Resolution: 2.70 Å
  • Aggregation State: PARTICLE 
  • Reconstruction Method: SINGLE PARTICLE 
EM Software:
TaskSoftware PackageVersion
MODEL REFINEMENTServalcat
RECONSTRUCTIONcryoSPARC

Structure Validation

View Full Validation Report



Entry History & Funding Information

Deposition Data


Funding OrganizationLocationGrant Number
Japan Society for the Promotion of Science (JSPS)Japan23KJ0720
Japan Society for the Promotion of Science (JSPS)Japan21H05281
Japan Society for the Promotion of Science (JSPS)Japan22H00403

Revision History  (Full details and data files)

  • Version 1.0: 2025-11-12
    Type: Initial release