Download MendeleyCrystal structure of Ta_Cel5A E133Q Y200F variant in complex with cellopentaose
Dutoit, R.To be published.
9F0M | pdb_00009f0m
Crystal structure of Ta_Cel5A E133Q Y200F variant in complex with cellopentaose
- PDB DOI: https://doi.org/10.2210/pdb9F0M/pdb
- Classification: HYDROLASE
- Organism(s): Thermoascus aurantiacus ATCC 26904
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2024-04-17 Released: 2025-04-30
- Funding Organization(s): Other government
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.63 Å
- R-Value Free: 0.174 (Depositor), 0.172 (DCC)
- R-Value Work: 0.143 (Depositor), 0.141 (DCC)
- R-Value Observed: 0.144 (Depositor)
Starting Model: experimental
View more details
wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| cellulase | 306 | Thermoascus aurantiacus ATCC 26904 | Mutation(s): 2 Gene Names: eg1 EC: 3.2.1.4 |  | |
UniProt | |||||
Find proteins for Q8TG26 (Thermoascus aurantiacus) Explore Q8TG26 Go to UniProtKB: Q8TG26 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q8TG26 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Oligosaccharides
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| SO4 Query on SO4 | I [auth B], J [auth B] | SULFATE ION O4 S QAOWNCQODCNURD-UHFFFAOYSA-L |  | ||
| GOL Query on GOL | E [auth A], F [auth A], G [auth B], H [auth B] | GLYCEROL C3 H8 O3 PEDCQBHIVMGVHV-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.63 Å
- R-Value Free: 0.174 (Depositor), 0.172 (DCC)
- R-Value Work: 0.143 (Depositor), 0.141 (DCC)
- R-Value Observed: 0.144 (Depositor)
Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 76.39 | α = 90 |
| b = 85.42 | β = 90 |
| c = 89.31 | γ = 90 |
| Software Name | Purpose |
|---|---|
| PHENIX | refinement |
| XSCALE | data scaling |
| XDS | data reduction |
| PHASER | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2025-04-30 Deposition Author(s): Dutoit, R.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Other government | Belgium | BAG 20210875 |
Revision History (Full details and data files)
- Version 1.0: 2025-04-30
Type: Initial release
