4XI2 | pdb_00004xi2

Crystal Structure of an auto-inhibited form of Bruton's Tryrosine Kinase

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d4xi2a1	All beta proteins	SH3-like barrel	SH3-domain	automated matches	automated matches	Mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)
A	d4xi2a2	Alpha and beta proteins (a+b)	Protein kinase-like (PK-like)	Protein kinase-like (PK-like)	automated matches	automated matches	Mouse (Mus musculus ) [TaxId: 10090 ],	SCOPe (2.08)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	SH3_1	e4xi2A2	A: beta barrels	X: SH3	H: SH3	T: SH3	F: SH3_1	ECOD (1.6)
A	SH2	e4xi2A1	A: a+b two layers	X: SH2 (From Topology)	H: SH2 (From Topology)	T: SH2	F: SH2	ECOD (1.6)
A	Pkinase_Tyr	e4xi2A3	A: a+b complex topology	X: Protein kinase/SAICAR synthase/ATP-grasp (From Homology)	H: Protein kinase/SAICAR synthase/ATP-grasp	T: Protein kinase	F: Pkinase_Tyr	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.30.30.40	Mainly Beta	Roll	SH3 type barrels.	SH3 Domains	CATH (utative)
A	3.30.200.20	Alpha Beta	2-Layer Sandwich	Phosphorylase Kinase	domain 1	CATH (utative)
A	1.10.510.10	Mainly Alpha	Orthogonal Bundle	Transferase(Phosphotransferase)	domain 1	CATH (utative)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF00017	SH2 domain (SH2)	SH2 domain		Domain
A	PF00018	SH3 domain (SH3_1)	SH3 domain	SH3 (Src homology 3) domains are often indicative of a protein involved in signal transduction related to cytoskeletal organisation. First described in the Src cytoplasmic tyrosine kinase Swiss:P12931. The structure is a partly opened beta barrel.	Domain
A	PF07714	Protein tyrosine and serine/threonine kinase (PK_Tyr_Ser-Thr)	Protein tyrosine and serine/threonine kinase	Protein phosphorylation, which plays a key role in most cellular activities, is a reversible process mediated by protein kinases and phosphoprotein phosphatases. Protein kinases catalyse the transfer of the gamma phosphate from nucleotide triphospha ...	Protein phosphorylation, which plays a key role in most cellular activities, is a reversible process mediated by protein kinases and phosphoprotein phosphatases. Protein kinases catalyse the transfer of the gamma phosphate from nucleotide triphosphates (often ATP) to one or more amino acid residues in a protein substrate side chain, resulting in a conformational change affecting protein function. Phosphoprotein phosphatases catalyse the reverse process. Protein kinases fall into three broad classes, characterised with respect to substrate specificity [1]; Serine/threonine-protein kinases, tyrosine-protein kinases, and dual specificity protein kinases (e.g. MEK - phosphorylates both Thr and Tyr on target proteins). This entry represents the catalytic domain found in a number of serine/threonine- and tyrosine-protein kinases. It does not include the catalytic domain of dual specificity kinases.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Tyrosine-protein kinase BTK	protein tyrosine kinase activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding protein tyrosine kinase activity catalytic activity, acting on a protein kinase activity protein kinase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding non-membrane spanning protein tyrosine kinase activity identical protein binding protein binding phospholipase binding enzyme binding phosphatidylinositol-3,4,5-trisphosphate binding phospholipid binding lipid binding phosphatidylinositol phosphate binding phosphatidylinositol binding enzyme regulator activity molecular function activator activity hydrolase activity phospholipase activity lipase activity enzyme activator activity hydrolase activity, acting on ester bonds phospholipase activator activity molecular function regulator activity lipase activator activity cation binding transition metal ion binding zinc ion binding metal ion binding	negative regulation of lymphocyte proliferation multicellular organismal process regulation of cellular process regulation of leukocyte activation negative regulation of biological process regulation of cell activation lymphocyte activation negative regulation of lymphocyte activation leukocyte proliferation leukocyte activation negative regulation of mononuclear cell proliferation negative regulation of leukocyte proliferation regulation of B cell proliferation lymphocyte proliferation regulation of B cell activation negative regulation of lymphocyte proliferation multicellular organismal process regulation of cellular process regulation of leukocyte activation negative regulation of biological process regulation of cell activation lymphocyte activation negative regulation of lymphocyte activation leukocyte proliferation leukocyte activation negative regulation of mononuclear cell proliferation negative regulation of leukocyte proliferation regulation of B cell proliferation lymphocyte proliferation regulation of B cell activation negative regulation of cellular process regulation of immune system process regulation of multicellular organismal process negative regulation of B cell activation regulation of lymphocyte proliferation regulation of leukocyte proliferation regulation of biological process negative regulation of cell population proliferation cell population proliferation B cell activation biological regulation cell activation B cell proliferation negative regulation of B cell proliferation negative regulation of cell activation negative regulation of immune system process regulation of cell population proliferation mononuclear cell proliferation immune system process regulation of mononuclear cell proliferation cellular process negative regulation of multicellular organismal process negative regulation of leukocyte activation regulation of lymphocyte activation positive regulation of cell population proliferation positive regulation of epithelial cell proliferation positive regulation of biological process regulation of synoviocyte proliferation regulation of epithelial cell proliferation synoviocyte proliferation epithelial cell proliferation positive regulation of synoviocyte proliferation positive regulation of cellular process carbohydrate derivative catabolic process glycoprotein catabolic process catabolic process carbohydrate derivative metabolic process primary metabolic process protein catabolic process glycoprotein metabolic process macromolecule catabolic process metabolic process protein metabolic process macromolecule metabolic process proteoglycan catabolic process proteoglycan metabolic process apoptotic process cell death programmed cell death biological process involved in interspecies interaction between organisms response to stimulus immune response response to other organism defense response defense response to symbiont response to external stimulus response to biotic stimulus defense response to other organism response to stress innate immune response response to external biotic stimulus establishment of organelle localization regulated exocytosis nitrogen compound transport cell activation involved in immune response leukocyte degranulation vesicle-mediated transport myeloid leukocyte activation myeloid leukocyte mediated immunity cell communication localization establishment of localization in cell leukocyte mediated immunity regulation of biological quality regulation of hormone levels histamine secretion by mast cell vacuolar localization establishment of localization mast cell degranulation immune effector process histamine secretion involved in inflammatory response exocytosis lysosome localization inflammatory response histamine production involved in inflammatory response cellular localization transport signaling hormone secretion histamine secretion secretion by cell organelle localization signal release leukocyte activation involved in immune response histamine transport mast cell activation mast cell mediated immunity production of molecular mediator involved in inflammatory response secretion export from cell cell-cell signaling myeloid cell activation involved in immune response mast cell activation involved in immune response exocytic process hormone transport regulation of localization import into cell positive regulation of cellular component organization regulation of cellular component organization cellular component organization positive regulation of endocytosis phagocytosis cellular component organization or biogenesis regulation of endocytosis regulation of transport regulation of phagocytosis regulation of vesicle-mediated transport positive regulation of phagocytosis positive regulation of transport endocytosis cellular response to stimulus cellular response to molecule of fungal origin cellular response to biotic stimulus response to molecule of fungal origin response to fungus cytokine production regulation of cytokine production negative regulation of macromolecule metabolic process regulation of biosynthetic process negative regulation of metabolic process negative regulation of cytokine production regulation of macromolecule metabolic process gene expression macromolecule biosynthetic process regulation of gene expression negative regulation of gene expression biosynthetic process negative regulation of biosynthetic process negative regulation of macromolecule biosynthetic process regulation of macromolecule biosynthetic process regulation of metabolic process positive regulation of macromolecule biosynthetic process positive regulation of metabolic process positive regulation of biosynthetic process positive regulation of macromolecule metabolic process interleukin-17 production regulation of interleukin-17A production regulation of interleukin-17 production positive regulation of interleukin-17A production positive regulation of gene expression positive regulation of multicellular organismal process positive regulation of interleukin-17 production interleukin-17A production positive regulation of cytokine production regulation of immune effector process immunoglobulin production positive regulation of immune effector process positive regulation of immune system process positive regulation of immunoglobulin production production of molecular mediator of immune response regulation of immunoglobulin production positive regulation of production of molecular mediator of immune response regulation of production of molecular mediator of immune response positive regulation of B cell proliferation positive regulation of lymphocyte activation positive regulation of cell activation positive regulation of leukocyte activation positive regulation of mononuclear cell proliferation positive regulation of lymphocyte proliferation positive regulation of B cell activation positive regulation of leukocyte proliferation monocyte proliferation positive regulation of cell communication positive regulation of response to biotic stimulus positive regulation of signal transduction pattern recognition receptor signaling pathway regulation of signal transduction intracellular signal transduction cGAS/STING signaling pathway immune response-activating signaling pathway positive regulation of immune response signal transduction innate immune response-activating signaling pathway positive regulation of pattern recognition receptor signaling pathway regulation of innate immune response positive regulation of intracellular signal transduction positive regulation of cGAS/STING signaling pathway regulation of response to stimulus regulation of pattern recognition receptor signaling pathway positive regulation of defense response activation of innate immune response regulation of immune response activation of immune response cytoplasmic pattern recognition receptor signaling pathway regulation of defense response regulation of response to biotic stimulus regulation of response to external stimulus intracellular receptor signaling pathway regulation of cytoplasmic pattern recognition receptor signaling pathway positive regulation of response to stimulus positive regulation of response to external stimulus immune response-regulating signaling pathway regulation of intracellular signal transduction regulation of response to stress regulation of signaling positive regulation of innate immune response regulation of cell communication positive regulation of signaling adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains positive regulation of acute inflammatory response to antigenic stimulus lymphocyte mediated immunity regulation of acute inflammatory response to antigenic stimulus positive regulation of leukocyte mediated immunity inflammatory response to antigenic stimulus positive regulation of hypersensitivity positive regulation of inflammatory response positive regulation of adaptive immune response positive regulation of immunoglobulin mediated immune response hypersensitivity regulation of adaptive immune response regulation of inflammatory response regulation of B cell mediated immunity regulation of leukocyte mediated immunity B cell mediated immunity regulation of acute inflammatory response positive regulation of type I hypersensitivity regulation of type I hypersensitivity positive regulation of adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains positive regulation of lymphocyte mediated immunity positive regulation of acute inflammatory response positive regulation of B cell mediated immunity type I hypersensitivity regulation of adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains regulation of immunoglobulin mediated immune response acute inflammatory response regulation of hypersensitivity regulation of inflammatory response to antigenic stimulus acute inflammatory response to antigenic stimulus positive regulation of inflammatory response to antigenic stimulus adaptive immune response immunoglobulin mediated immune response regulation of lymphocyte mediated immunity immune response-regulating cell surface receptor signaling pathway cell surface receptor signaling pathway immune response-activating cell surface receptor signaling pathway B cell receptor signaling pathway antigen receptor-mediated signaling pathway regulation of myeloid leukocyte mediated immunity regulation of type III hypersensitivity positive regulation of myeloid leukocyte mediated immunity type III hypersensitivity positive regulation of type III hypersensitivity interleukin-6 production positive regulation of interleukin-6 production regulation of interleukin-6 production cellular developmental process immunoglobulin production involved in immunoglobulin-mediated immune response cell development mononuclear cell differentiation B cell affinity maturation lymphocyte differentiation B cell differentiation cell differentiation anatomical structure development developmental process hemopoiesis peripheral B cell selection B cell selection leukocyte differentiation negative regulation of interleukin-10 production interleukin-10 production regulation of interleukin-10 production anatomical structure maturation cell maturation developmental maturation regulation of NLRP3 inflammasome complex assembly positive regulation of NLRP3 inflammasome complex assembly cellular component biogenesis cellular component assembly inflammasome-mediated signaling pathway canonical inflammasome complex assembly positive regulation of inflammasome-mediated signaling pathway regulation of inflammasome-mediated signaling pathway NLRP3 inflammasome complex assembly regulation of protein-containing complex assembly positive regulation of protein-containing complex assembly protein-containing complex assembly protein-containing complex organization positive regulation of cellular component biogenesis regulation of cellular component biogenesis cellular response to oxidative stress response to oxygen-containing compound cellular response to chemical stress cellular response to oxygen-containing compound response to reactive oxygen species cellular response to reactive oxygen species response to oxidative stress cellular response to chemical stimulus cellular response to stress response to chemical multicellular organismal-level homeostasis leukocyte homeostasis homeostatic process homeostasis of number of cells myeloid cell homeostasis neutrophil homeostasis eosinophil homeostasis regulation of tumor necrosis factor production positive regulation of tumor necrosis factor superfamily cytokine production tumor necrosis factor superfamily cytokine production positive regulation of tumor necrosis factor production tumor necrosis factor production regulation of tumor necrosis factor superfamily cytokine production response to lipid response to lipopolysaccharide response to bacterium response to molecule of bacterial origin response to peptide response to interleukin-7 response to cytokine cellular response to cytokine stimulus cellular response to interleukin-7	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol perinuclear region of cytoplasm cell periphery plasma membrane membrane membrane microdomain membrane raft membrane-bounded organelle intracellular organelle cytoplasmic vesicle organelle cellular anatomical structure intracellular anatomical structure cytoplasm cytosol perinuclear region of cytoplasm cell periphery plasma membrane membrane membrane microdomain membrane raft membrane-bounded organelle intracellular organelle cytoplasmic vesicle organelle intracellular vesicle intracellular membrane-bounded organelle vesicle nucleus

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR008266	Tyrosine-protein kinase, active site	Active Site
A	IPR020635	Tyrosine-protein kinase, catalytic domain	Domain
A	IPR035574	Tyrosine-protein kinase BTK, SH3 domain	Domain
A	IPR036028	SH3-like domain superfamily	Homologous Superfamily
A	IPR000719	Protein kinase domain	Domain
A	IPR001562	Zinc finger, Btk motif	Conserved Site
A	IPR001452	SH3 domain	Domain
A	IPR017441	Protein kinase, ATP binding site	Binding Site
A	IPR001849	Pleckstrin homology domain	Domain
A	IPR036860	SH2 domain superfamily	Homologous Superfamily
A	IPR000980	SH2 domain	Domain
A	IPR011993	PH-like domain superfamily	Homologous Superfamily
A	IPR001245	Serine-threonine/tyrosine-protein kinase, catalytic domain	Domain
A	IPR011009	Protein kinase-like domain superfamily	Homologous Superfamily
A	IPR050198	Non-receptor tyrosine kinases involved in cell signaling	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.