5AZA | pdb_00005aza

Crystal structure of MBP-sAglB fusion protein with a 20-residue spacer in the connector helix

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Type 2 solute binding protein-like	8058187	3000083	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	EUF07568	e5azaA7	A: beta barrels	X: STT3/PglB/AglB beta-barrel domain (From Topology)	H: STT3/PglB/AglB beta-barrel domain (From Topology)	T: STT3/PglB/AglB beta-barrel domain	F: EUF07568	ECOD (1.6)
A	EUF07789	e5azaA6	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Ig-like domains in STT3 (From Topology)	T: Ig-like domains in STT3	F: EUF07789	ECOD (1.6)
A	EUF07790	e5azaA2	A: beta sandwiches	X: Immunoglobulin-like beta-sandwich	H: Ig-like domains in STT3 (From Topology)	T: Ig-like domains in STT3	F: EUF07790	ECOD (1.6)
A	archaeo_STT3_1st	e5azaA5	A: a/b three-layered sandwiches	X: STT3/PglB/AglB core domain (From Topology)	H: STT3/PglB/AglB core domain (From Topology)	T: STT3/PglB/AglB core domain	F: archaeo_STT3_1st	ECOD (1.6)
A	SBP_bac_1_C	e5azaA1	A: a/b three-layered sandwiches	X: Periplasmic binding protein-like II (From Topology)	H: Periplasmic binding protein-like II (From Topology)	T: Periplasmic binding protein-like II	F: SBP_bac_1_C	ECOD (1.6)
A	SBP_bac_1_N_1	e5azaA3	A: a/b three-layered sandwiches	X: Periplasmic binding protein-like II (From Topology)	H: Periplasmic binding protein-like II (From Topology)	T: Periplasmic binding protein-like II	F: SBP_bac_1_N_1	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.60.40.3020	Mainly Beta	Sandwich	Immunoglobulin-like		CATH (utative)
A	2.60.40.3030	Mainly Beta	Sandwich	Immunoglobulin-like		CATH (utative)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF01547	Bacterial extracellular solute-binding protein (SBP_bac_1)	Bacterial extracellular solute-binding protein	-	Family
A	PF18246	Oligosaccharyltransferase Insert domain (OST_IS)	Oligosaccharyltransferase Insert domain	This is a domain found in STT3 present in P. furiosus. In P. furiousus, STT3 is an Oligosaccharyltransferase which catalyzes the transfer of a heptasaccharide, containing one hexouronate and two pentose residues, onto peptides in an Asn-X-Thr/Ser-mot ...	This is a domain found in STT3 present in P. furiosus. In P. furiousus, STT3 is an Oligosaccharyltransferase which catalyzes the transfer of a heptasaccharide, containing one hexouronate and two pentose residues, onto peptides in an Asn-X-Thr/Ser-motif-dependent manner. This domain is inserted into the central core (CC) domain and hence is referred to as the Insert (IS) domain. This IS domain contains a disulphide bond [1].	Domain
A	PF21618	Oligosaccharyl transferase, Peripheral 1 domain (OST_P1)	Oligosaccharyl transferase, Peripheral 1 domain	This domain is found in Oligosaccharyl transferase from Pyrococcus furiosus (OST, also known as AglB-L) and similar archaeal sequences. OST catalyses the co-translational transfer of an oligosaccharide from a lipid donor to an asparagine residue in n ...	This domain is found in Oligosaccharyl transferase from Pyrococcus furiosus (OST, also known as AglB-L) and similar archaeal sequences. OST catalyses the co-translational transfer of an oligosaccharide from a lipid donor to an asparagine residue in nascent polypeptide chains. This protein is organised into four regions: a N-terminal half (Pfam:PF02516), the central core (CC) domain that includes the IS (insert) domain (Pfam:PF18246) and two peripheral domains, P1 (this entry) and P2 (Pfam:PF18235). This domain, which consists mainly of beta-strands, encircles the central core domain [1].	Domain
A	PF18235	Oligosaccharyltransferase Peripheral 2 domain (OST_P2)	Oligosaccharyltransferase Peripheral 2 domain	This is a domain found in the C-terminal region of STT3 present in P. furiosus. STT3 is an Oligosaccharyltransferase which catalyzes the transfer of a heptasaccharide, containing one hexouronate and two pentose residues, onto peptides in an Asn-X-Thr ...	This is a domain found in the C-terminal region of STT3 present in P. furiosus. STT3 is an Oligosaccharyltransferase which catalyzes the transfer of a heptasaccharide, containing one hexouronate and two pentose residues, onto peptides in an Asn-X-Thr/Ser-motif-dependent manner. This domain, known as the Peripheral 2 (P2) domain, encircles the central core domain [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Maltose-binding periplasmic protein,Oligosaccharyl transferase stt3 subunit related protein	oligosaccharyl transferase activity glycosyltransferase activity transferase activity hexosyltransferase activity catalytic activity cation binding ion binding binding metal ion binding small molecule binding transporter activity carbohydrate transmembrane transporter activity transmembrane transporter activity oligosaccharide binding oligosaccharyl transferase activity glycosyltransferase activity transferase activity hexosyltransferase activity catalytic activity cation binding ion binding binding metal ion binding small molecule binding transporter activity carbohydrate transmembrane transporter activity transmembrane transporter activity oligosaccharide binding disaccharide binding carbohydrate binding maltose binding	carbohydrate derivative biosynthetic process glycoprotein biosynthetic process carbohydrate derivative metabolic process primary metabolic process glycoprotein metabolic process protein glycosylation macromolecule biosynthetic process biosynthetic process glycosylation macromolecule modification macromolecule glycosylation metabolic process cellular process protein metabolic process carbohydrate derivative biosynthetic process glycoprotein biosynthetic process carbohydrate derivative metabolic process primary metabolic process glycoprotein metabolic process protein glycosylation macromolecule biosynthetic process biosynthetic process glycosylation macromolecule modification macromolecule glycosylation metabolic process cellular process protein metabolic process macromolecule metabolic process protein modification process cellular response to stimulus cellular response to stress response to stimulus DNA damage response response to stress cell chemotaxis chemotaxis response to external stimulus cellular response to chemical stimulus taxis response to chemical cell migration cell motility locomotion localization transport carbohydrate transport oligosaccharide transport disaccharide transport establishment of localization maltose transport polysaccharide localization dextrin transport macromolecule localization maltodextrin transmembrane transport polysaccharide transport response to disaccharide response to oxygen-containing compound detection of maltose stimulus detection of stimulus response to carbohydrate detection of chemical stimulus detection of disaccharide stimulus response to maltose detection of carbohydrate stimulus	cell periphery cellular anatomical structure plasma membrane membrane ATPase dependent transmembrane transport complex protein-containing complex plasma membrane protein complex membrane protein complex transporter complex ATP-binding cassette (ABC) transporter complex transmembrane transporter complex ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing maltose transport complex periplasmic space cell periphery cellular anatomical structure plasma membrane membrane ATPase dependent transmembrane transport complex protein-containing complex plasma membrane protein complex membrane protein complex transporter complex ATP-binding cassette (ABC) transporter complex transmembrane transporter complex ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing maltose transport complex periplasmic space outer membrane-bounded periplasmic space cell envelope

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR003674	Oligosaccharyl transferase, STT3 subunit	Family
A	IPR048307	Oligosaccharyl transferase STT3, N-terminal domain	Domain
A	IPR041152	Oligosaccharyltransferase, peripheral 2 domain	Domain
A	IPR048858	Oligosaccharyl transferase, peripheral 1 domain	Domain
A	IPR041530	Oligosaccharyltransferase insert domain	Domain
A	IPR006061	Solute-binding family 1, conserved site	Conserved Site
A	IPR006060	Maltose/Cyclodextrin ABC transporter, substrate-binding protein	Family
A	IPR006059	Bacterial-type extracellular solute-binding protein	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.