6LPF | pdb_00006lpf

The crystal structure of human cytoplasmic LRS

External Resource: Annotation

Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	tRNA-synt_1_2	e6lpfB1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: acid protease	F: tRNA-synt_1_2	ECOD (1.6)
B	UNK_F_TYPE	e6lpfB5	A: alpha arrays	X: inserted region in the C-terminal domain of the archeal LeuRS (From Topology)	H: inserted region in the C-terminal domain of the archeal LeuRS (From Topology)	T: inserted region in the C-terminal domain of the archeal LeuRS	F: UNK_F_TYPE	ECOD (1.6)
B	Anticodon_1,tRNA-synt_1g	e6lpfB3	A: alpha bundles	X: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases (From Topology)	H: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases (From Topology)	T: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases	F: Anticodon_1,tRNA-synt_1g	ECOD (1.6)
B	UNK_F_TYPE	e6lpfB6	A: a+b three layers	X: A subdomain in the anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases (From Topology)	H: A subdomain in the anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases (From Topology)	T: A subdomain in the anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases	F: UNK_F_TYPE	ECOD (1.6)
B	tRNA-synt_1_1st	e6lpfB2	A: a/b three-layered sandwiches	X: HUP domain-like	H: HUP domains (From Topology)	T: HUP domains	F: tRNA-synt_1_1st	ECOD (1.6)
B	leuS_1st	e6lpfB4	A: few secondary structure elements	X: Rubredoxin-like	H: Rubredoxin-related	T: Rubredoxin-related	F: leuS_1st	ECOD (1.6)
A	tRNA-synt_1_2	e6lpfA1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: acid protease	F: tRNA-synt_1_2	ECOD (1.6)
A	UNK_F_TYPE	e6lpfA5	A: alpha arrays	X: inserted region in the C-terminal domain of the archeal LeuRS (From Topology)	H: inserted region in the C-terminal domain of the archeal LeuRS (From Topology)	T: inserted region in the C-terminal domain of the archeal LeuRS	F: UNK_F_TYPE	ECOD (1.6)
A	Anticodon_1,tRNA-synt_1g	e6lpfA3	A: alpha bundles	X: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases (From Topology)	H: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases (From Topology)	T: Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases	F: Anticodon_1,tRNA-synt_1g	ECOD (1.6)
A	UNK_F_TYPE	e6lpfA6	A: a+b three layers	X: A subdomain in the anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases (From Topology)	H: A subdomain in the anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases (From Topology)	T: A subdomain in the anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases	F: UNK_F_TYPE	ECOD (1.6)
A	tRNA-synt_1_1st	e6lpfA2	A: a/b three-layered sandwiches	X: HUP domain-like	H: HUP domains (From Topology)	T: HUP domains	F: tRNA-synt_1_1st	ECOD (1.6)
A	leuS_1st	e6lpfA4	A: few secondary structure elements	X: Rubredoxin-like	H: Rubredoxin-related	T: Rubredoxin-related	F: leuS_1st	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.730.20	Mainly Alpha	Orthogonal Bundle	Isoleucyl-tRNA Synthetase	Domain 1	CATH (utative)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF08264	Anticodon-binding domain of tRNA ligase (Anticodon_1)	Anticodon-binding domain of tRNA ligase	This domain is found mainly hydrophobic tRNA synthetases. The domain binds to the anticodon of the tRNA ligase.	Domain
A, B	PF00133	tRNA synthetases class I (I, L, M and V) (tRNA-synt_1)	tRNA synthetases class I (I, L, M and V)	Other tRNA synthetase sub-families are too dissimilar to be included.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Leucine--tRNA ligase, cytoplasmic	ligase activity, forming carbon-oxygen bonds aminoacyl-tRNA ligase activity catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid leucine-tRNA ligase activity catalytic activity, acting on a tRNA catalytic activity ligase activity GTPase regulator activity enzyme regulator activity enzyme activator activity GTPase activator activity molecular function activator activity nucleoside-triphosphatase regulator activity ligase activity, forming carbon-oxygen bonds aminoacyl-tRNA ligase activity catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid leucine-tRNA ligase activity catalytic activity, acting on a tRNA catalytic activity ligase activity GTPase regulator activity enzyme regulator activity enzyme activator activity GTPase activator activity molecular function activator activity nucleoside-triphosphatase regulator activity molecular function regulator activity binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding glutamine-tRNA ligase activity aminoacyl-tRNA deacylase activity hydrolase activity, acting on ester bonds deacylase activity carboxylic ester hydrolase activity hydrolase activity	cellular component organization regulation of cellular component size biological regulation cellular process cellular component organization or biogenesis regulation of biological quality regulation of cell size regulation of anatomical structure size cellular response to stimulus positive regulation of cell communication regulation of cellular process positive regulation of signal transduction response to stimulus signaling cellular component organization regulation of cellular component size biological regulation cellular process cellular component organization or biogenesis regulation of biological quality regulation of cell size regulation of anatomical structure size cellular response to stimulus positive regulation of cell communication regulation of cellular process positive regulation of signal transduction response to stimulus signaling positive regulation of biological process regulation of signal transduction intracellular signal transduction cell communication positive regulation of response to stimulus regulation of intracellular signal transduction positive regulation of TOR signaling signal transduction positive regulation of cellular process TOR signaling regulation of biological process regulation of signaling regulation of TOR signaling positive regulation of intracellular signal transduction regulation of response to stimulus regulation of cell communication positive regulation of signaling positive regulation of molecular function regulation of molecular function positive regulation of hydrolase activity regulation of catalytic activity regulation of hydrolase activity positive regulation of catalytic activity regulation of GTPase activity positive regulation of GTPase activity response to nutrient levels cellular response to leucine starvation cellular response to amino acid starvation cellular response to starvation cellular response to nutrient levels cellular response to stress response to stress response to amino acid starvation response to starvation amino acid activation tRNA aminoacylation RNA metabolic process primary metabolic process amino acid metabolic process translation gene expression tRNA aminoacylation for protein translation protein biosynthetic process macromolecule biosynthetic process nucleic acid metabolic process tRNA metabolic process nucleobase-containing compound metabolic process biosynthetic process metabolic process macromolecule metabolic process protein metabolic process positive regulation of TORC1 signaling regulation of TORC1 signaling TORC1 signaling cellular response to acid chemical cellular response to amino acid stimulus response to acid chemical cellular response to chemical stimulus response to chemical response to amino acid leucyl-tRNA aminoacylation glutaminyl-tRNA aminoacylation cellular response to nitrogen compound response to oxygen-containing compound response to nitrogen compound cellular response to L-leucine cellular response to oxygen-containing compound response to L-leucine	cellular anatomical structure endomembrane system intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle organelle lytic vacuole vacuole intracellular membrane-bounded organelle lysosome protein-containing complex catalytic complex cellular anatomical structure endomembrane system intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle organelle lytic vacuole vacuole intracellular membrane-bounded organelle lysosome protein-containing complex catalytic complex aminoacyl-tRNA synthetase multienzyme complex intracellular protein-containing complex nucleus membrane-enclosed lumen intracellular membraneless organelle intracellular organelle lumen nuclear body nucleoplasm organelle lumen membraneless organelle nuclear lumen endoplasmic reticulum

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR001412	Aminoacyl-tRNA synthetase, class I, conserved site	Conserved Site
A, B	IPR055416	Leucine--tRNA ligase, RagD-binding domain	Domain
A, B	IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	Homologous Superfamily
A, B	IPR054509	Leucine--tRNA ligase, ubiquitin-like domain	Domain
A, B	IPR002300	Aminoacyl-tRNA synthetase, class Ia	Domain
A, B	IPR004493	Leucyl-tRNA synthetase, class Ia, archaeal/eukaryotic cytosolic	Family
A, B	IPR009080	Aminoacyl-tRNA synthetase, class Ia, anticodon-binding	Homologous Superfamily
A, B	IPR009008	Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain	Homologous Superfamily
A, B	IPR013155	Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: Q9P2J5	infantile liver failure syndrome 1 astrocytic tumor Larsen syndrome myopathy tuberculosis malignant ependymoma oligodendroglioma large cell medulloblastoma medulloblastoma ovarian cancer

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.