NEUTRON DIFFRACTION
Starting Model(s)
Initial Refinement Model(s) | |||
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Type | Source | Accession Code | Details |
experimental model | PDB | 5H59 |
Crystallization
Crystalization Experiments | ||||
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ID | Method | pH | Temperature | Details |
1 | BATCH MODE | 293.15 | PEG2000 |
Crystal Data
Unit Cell | |
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Length ( Å ) | Angle ( ˚ ) |
a = 59.32 | α = 90 |
b = 59.32 | β = 90 |
c = 187.69 | γ = 120 |
Symmetry | |
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Space Group | P 32 2 1 |
Diffraction
Diffraction Experiment | ||||||||||||||
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ID # | Crystal ID | Scattering Type | Data Collection Temperature | Detector | Detector Type | Details | Collection Date | Monochromator | Protocol | |||||
1 | 1 | neutron | 100 | DIFFRACTOMETER | iBIX | 2022-12-10 | L | LAUE |
Radiation Source | |||||
---|---|---|---|---|---|
ID # | Source | Type | Wavelength List | Synchrotron Site | Beamline |
1 | SPALLATION SOURCE | J-PARC MLF BEAMLINE BL-03 | 2.05-5.38 | JPARC MLF | BL-03 |
Data Collection
Overall | |||||||||||||||||||
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ID # | Resolution (High) | Resolution (Low) | Percent Possible (Observed) | Rpim I (All) | CC (Half) | Net I Over Average Sigma (I) | Redundancy | Number Reflections (All) | Number Reflections (Observed) | Observed Criterion Sigma (F) | Observed Criterion Sigma (I) | B (Isotropic) From Wilson Plot | |||||||
1 | 1.8 | 19.425 | 99.9 | 0.1669 | 0.9613 | 5.41 | 9.5293 | 36398 |
Highest Resolution Shell | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ID # | Resolution (High) | Resolution (Low) | Percent Possible (All) | Percent Possible (Observed) | Rpim I (All) | CC (Half) | Mean I Over Sigma (Observed) | Redundancy | Number Unique Reflections (All) | ||||||||||
1 | 1.8 | 1.86 | 0.7279 | 0.3668 |
Refinement
Statistics | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Diffraction ID | Structure Solution Method | Cross Validation method | Starting model | Resolution (High) | Resolution (Low) | Number Reflections (Observed) | Number Reflections (R-Free) | Percent Reflections (Observed) | R-Factor (All) | R-Work (Depositor) | R-Free (Depositor) | Mean Isotropic B | |||||||
NEUTRON DIFFRACTION | MOLECULAR REPLACEMENT | FREE R-VALUE | 5H59 | 1.8 | 19.425 | 36397 | 1771 | 99.66 | 0.199 | 0.1971 | 0.2462 | 22.416 |
Temperature Factor Modeling | ||||||
---|---|---|---|---|---|---|
Anisotropic B[1][1] | Anisotropic B[1][2] | Anisotropic B[1][3] | Anisotropic B[2][2] | Anisotropic B[2][3] | Anisotropic B[3][3] | |
-0.027 | -0.014 | -0.027 | 0.089 |
RMS Deviations | |
---|---|
Key | Refinement Restraint Deviation |
r_dihedral_angle_6_deg | 16.538 |
r_dihedral_angle_3_deg | 15.5 |
r_dihedral_angle_other_6_deg | 13.899 |
r_dihedral_angle_2_deg | 7.013 |
r_dihedral_angle_1_deg | 6.344 |
r_lrange_it | 5.09 |
r_lrange_other | 4.794 |
r_dihedral_angle_other_3_deg | 3.796 |
r_scangle_other | 3.575 |
r_scangle_it | 3.414 |
Software
Software | |
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Software Name | Purpose |
STARGazer | data reduction |
STARGazer | data scaling |
PHASER | phasing |
REFMAC | refinement |