NEUTRON DIFFRACTION
Starting Model(s)
Initial Refinement Model(s) | |||
---|---|---|---|
Type | Source | Accession Code | Details |
experimental model | PDB | 5H59 |
Crystallization
Crystalization Experiments | ||||
---|---|---|---|---|
ID | Method | pH | Temperature | Details |
1 | BATCH MODE | 293.15 | PEG2000 |
Crystal Data
Unit Cell | |
---|---|
Length ( Å ) | Angle ( ˚ ) |
a = 59.29 | α = 90 |
b = 59.29 | β = 90 |
c = 187.24 | γ = 120 |
Symmetry | |
---|---|
Space Group | P 32 2 1 |
Diffraction
Diffraction Experiment | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ID # | Crystal ID | Scattering Type | Data Collection Temperature | Detector | Detector Type | Details | Collection Date | Monochromator | Protocol | |||||
1 | 1 | neutron | 100 | DIFFRACTOMETER | iBIX | 2022-03-02 | L | LAUE |
Radiation Source | |||||
---|---|---|---|---|---|
ID # | Source | Type | Wavelength List | Synchrotron Site | Beamline |
1 | SPALLATION SOURCE | J-PARC MLF BEAMLINE BL-03 | 2.05-5.38 | JPARC MLF | BL-03 |
Data Collection
Overall | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ID # | Resolution (High) | Resolution (Low) | Percent Possible (Observed) | Rpim I (All) | CC (Half) | Net I Over Average Sigma (I) | Redundancy | Number Reflections (All) | Number Reflections (Observed) | Observed Criterion Sigma (F) | Observed Criterion Sigma (I) | B (Isotropic) From Wilson Plot | |||||||
1 | 1.8 | 19.415 | 99.5 | 0.1548 | 0.9682 | 6.34 | 9.0226 | 36250 |
Highest Resolution Shell | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
ID # | Resolution (High) | Resolution (Low) | Percent Possible (All) | Percent Possible (Observed) | Rpim I (All) | CC (Half) | Mean I Over Sigma (Observed) | Redundancy | Number Unique Reflections (All) | ||||||||||
1 | 1.8 | 1.86 | 0.718 | 0.3492 |
Refinement
Statistics | |||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Diffraction ID | Structure Solution Method | Cross Validation method | Starting model | Resolution (High) | Resolution (Low) | Number Reflections (Observed) | Number Reflections (R-Free) | Percent Reflections (Observed) | R-Factor (All) | R-Work (Depositor) | R-Free (Depositor) | Mean Isotropic B | |||||||
NEUTRON DIFFRACTION | MOLECULAR REPLACEMENT | FREE R-VALUE | 5H59 | 1.8 | 19.415 | 36248 | 1764 | 99.577 | 0.193 | 0.1904 | 0.2449 | 24.332 |
Temperature Factor Modeling | ||||||
---|---|---|---|---|---|---|
Anisotropic B[1][1] | Anisotropic B[1][2] | Anisotropic B[1][3] | Anisotropic B[2][2] | Anisotropic B[2][3] | Anisotropic B[3][3] | |
0.043 | 0.022 | 0.043 | -0.141 |
RMS Deviations | |
---|---|
Key | Refinement Restraint Deviation |
r_dihedral_angle_6_deg | 16.481 |
r_dihedral_angle_3_deg | 14.958 |
r_dihedral_angle_other_6_deg | 14.511 |
r_dihedral_angle_2_deg | 8.061 |
r_dihedral_angle_1_deg | 6.443 |
r_lrange_it | 5.377 |
r_lrange_other | 5.052 |
r_dihedral_angle_other_3_deg | 3.895 |
r_scangle_other | 3.895 |
r_scangle_it | 3.696 |
Software
Software | |
---|---|
Software Name | Purpose |
STARGazer | data reduction |
STARGazer | data scaling |
PHASER | phasing |
REFMAC | refinement |