Kojibiose hydrolase

UniProtKB accession:  A5FBJ5

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UniProtKB description:  Glycosidase that specifically hydrolyzes kojibiose to beta-glucose and glucose (PubMed:34728215). Also hydrolyzes, with lower catalytic efficiency, longer kojioligosaccharides (from kojitriose to kojipentaose) and shorter oligosaccharides produced by the degradation of dextran-containing alpha-1,2 branches (PubMed:34728215). Probably acts on alpha-(1->2)-glucosyl isomaltooligosaccharides (PubMed:37269952). Shows weak activity with nigerose but has no activity toward p-nitrophenyl alpha-glucopyranoside, which is a general substrate of exo-acting alpha-glucoside hydrolases (PubMed:34728215). Has a strict specificity for alpha-1,2-glucosidic linkages (PubMed:34728215). Catalyzes the hydrolytic reaction via an anomer-inverting mechanism (PubMed:34728215).