SAGA complex subunit SGF29

UniProtKB accession:  P25554

Grouped By:  Matching UniProtKB accession

UniProtKB description:  Chromatin reader component of the transcription coactivator SAGA complex. SAGA acts as a general cofactor required for essentially all RNA polymerase II transcription (PubMed:10026213, PubMed:10864329, PubMed:15647753, PubMed:25216679, PubMed:28918903). At the promoters, SAGA is required for transcription pre-initiation complex (PIC) recruitment. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (via core/TAF module) and promoter selectivity, interaction with transcription activators (via Tra1/SPT module), and chromatin modification through histone acetylation (via HAT module) and deubiquitination (via DUB module) (PubMed:22224423). SAGA preferentially acetylates histones H3 (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac) and H2B and deubiquitinates histone H2B (PubMed:10026213). SAGA interacts with DNA via upstream activating sequences (UASs) (PubMed:28918903). Also identified in a modified version of SAGA named SALSA or SLIK (PubMed:15647753). The cleavage of SPT7 and the absence of the SPT8 subunit in SLIK neither drive any major conformational differences in its structure compared with SAGA, nor significantly affect HAT, DUB, or DNA-binding activities (PubMed:33864814). SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (PubMed:21685874). Component of the ADA histone acetyltransferase complex, which preferentially acetylates nucleosomal histones H3 (to form H3K14ac and H3K18ac) and H2B (PubMed:10026213). SGF29 is also required for heterochromatin boundary formation function, prevention of the spread of gene silencing at the HMR locus (PubMed:24307402).