1TBD | pdb_00001tbd

Solution structure of the origin DNA-binding domain of SV40 T-antigen.

(1996) Nat Struct Biol 3: 1034-1039

• PubMed: 8946857 Search on PubMed
• DOI: https://doi.org/10.1038/nsb1296-1034
• Primary Citation of Related Structures:  
  1TBD, 2TBD


• PubMed Abstract: 
  

  The structure of the domain from simian virus 40 (SV40) large T-antigen that binds to the SV40 origin of DNA replication (T-ag-OBD131-260) has been determined by nuclear magnetic resonance spectroscopy. The overall fold, consisting of a central five-stranded antiparallel beta-sheet flanked by two alpha-helices on one side and one alpha-helix and one 3(10)-helix on the other, is a new one. Previous mutational analyses have identified two elements, termed A (approximately 152-155) and B2 (203-207), as essential for origin-specific recognition. These elements form two closely juxtaposed loops that define a continuous surface on the protein. The addition of a duplex oligonucleotide containing the origin recognition pentanucleotide GAGGC induces chemical shift changes and slows amide proton exchange in resonances from this region, indicating that this surface directly contacts the DNA.

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Organizational Affiliation: 
• Department of Biochemistry, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.