Download MendeleyThermus scotoductus SA-01 Ene-reductase triple mutant TsER_C25D_I67T_A102H
Opperman, D.J., Hoebenreich, S., Nett, N.To be published.
5OGT | pdb_00005ogt
Thermus scotoductus SA-01 Ene-reductase triple mutant TsER_C25D_I67T_A102H
- PDB DOI: https://doi.org/10.2210/pdb5OGT/pdb
- Classification: OXIDOREDUCTASE
- Organism(s): Thermus scotoductus SA-01
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2017-07-13 Released: 2018-08-01
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.30 Å
- R-Value Free: 0.244 (Depositor), 0.250 (DCC)
- R-Value Work: 0.204 (Depositor), 0.210 (DCC)
- R-Value Observed: 0.206 (Depositor)
Starting Model: experimental
View more details
This is version 1.1 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Chromate reductase | 349 | Thermus scotoductus SA-01 | Mutation(s): 3 Gene Names: chrR |  | |
UniProt | |||||
Find proteins for E8PRF1 (Thermus scotoductus (strain ATCC 700910 / SA-01)) Explore E8PRF1 Go to UniProtKB: E8PRF1 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | E8PRF1 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 1 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| FMN Query on FMN | E [auth A], F [auth B], G [auth C], H [auth D] | FLAVIN MONONUCLEOTIDE C17 H21 N4 O9 P FVTCRASFADXXNN-SCRDCRAPSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.30 Å
- R-Value Free: 0.244 (Depositor), 0.250 (DCC)
- R-Value Work: 0.204 (Depositor), 0.210 (DCC)
- R-Value Observed: 0.206 (Depositor)
Space Group: P 1 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 100.27 | α = 90 |
| b = 103.24 | β = 113.28 |
| c = 102.72 | γ = 90 |
| Software Name | Purpose |
|---|---|
| MOSFLM | data reduction |
| Aimless | data scaling |
| PHASER | phasing |
| REFMAC | refinement |
| PDB_EXTRACT | data extraction |
Entry History
Deposition Data
- Released Date: 2018-08-01 Deposition Author(s): Opperman, D.J., Hoebenreich, S., Nett, N.
Revision History (Full details and data files)
- Version 1.0: 2018-08-01
Type: Initial release - Version 1.1: 2024-01-17
Changes: Data collection, Database references, Refinement description
