Download MendeleyStructure of the LpMIP mutant D44E
Wiedemann, C., Whittaker, J.J., Guskov, A., Hellmich, U.A.To be published.
8RUO | pdb_00008ruo
D44E mutant crystal structure of LpMIP
- PDB DOI: https://doi.org/10.2210/pdb8RUO/pdb
- Classification: STRUCTURAL PROTEIN
- Organism(s): Legionella pneumophila
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2024-01-31 Released: 2025-02-12
- Funding Organization(s): German Research Foundation (DFG)
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.54 Å
- R-Value Free: 0.319 (Depositor), 0.325 (DCC)
- R-Value Work: 0.250 (Depositor), 0.269 (DCC)
- R-Value Observed: 0.254 (Depositor)
Starting Model: experimental
View more details
wwPDB Validation 3D Report Full Report
This is version 1.0 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Outer membrane protein MIP | 205 | Legionella pneumophila | Mutation(s): 1 Gene Names: mip EC: 5.2.1.8 |  | |
UniProt | |||||
Find proteins for Q70YI1 (Legionella pneumophila) Explore Q70YI1 Go to UniProtKB: Q70YI1 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q70YI1 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| MES Query on MES | B [auth A] | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID C6 H13 N O4 S SXGZJKUKBWWHRA-UHFFFAOYSA-N |  | ||
| PEG Query on PEG | C [auth A], D [auth A], E [auth A] | DI(HYDROXYETHYL)ETHER C4 H10 O3 MTHSVFCYNBDYFN-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.54 Å
- R-Value Free: 0.319 (Depositor), 0.325 (DCC)
- R-Value Work: 0.250 (Depositor), 0.269 (DCC)
- R-Value Observed: 0.254 (Depositor)
Space Group: P 43 21 2
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 77.185 | α = 90 |
| b = 77.185 | β = 90 |
| c = 103.517 | γ = 90 |
| Software Name | Purpose |
|---|---|
| REFMAC | refinement |
| XDS | data scaling |
| XDS | data reduction |
| MoRDa | phasing |
Entry History & Funding Information
Deposition Data
- Released Date: 2025-02-12 Deposition Author(s): Wiedemann, C., Whittaker, J.J., Guskov, A., Hellmich, U.A.
| Funding Organization | Location | Grant Number |
|---|---|---|
| German Research Foundation (DFG) | Germany | 390713860 |
Revision History (Full details and data files)
- Version 1.0: 2025-02-12
Type: Initial release
