Visualizing DNA polymerase iota catalyze Hoogsteen-directed DNA synthesis
Frevert, Z., Reusch, D.T., Gildenberg, M.S., Jordan, S.M., Ryan, B.J., Freudenthal, B.D., Washington, M.T.(2025) Nat Commun 16: 5979
Experimental Data Snapshot
Starting Model: experimental
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(2025) Nat Commun 16: 5979
Entity ID: 1 | |||||
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Molecule | Chains | Sequence Length | Organism | Details | Image |
DNA polymerase iota | 420 | Homo sapiens | Mutation(s): 0  Gene Names: POLI, RAD30B EC: 2.7.7.7 | ![]() | |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for Q9UNA4 (Homo sapiens) Explore Q9UNA4  Go to UniProtKB:  Q9UNA4 | |||||
PHAROS:  Q9UNA4 GTEx:  ENSG00000101751  | |||||
Entity Groups   | |||||
Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
UniProt Group | Q9UNA4 | ||||
Sequence AnnotationsExpand | |||||
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Find similar nucleic acids by: Sequence | 3D Structure
Entity ID: 2 | |||||
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Molecule | Chains | Length | Organism | Image | |
DNA (5'-D(P*AP*AP*GP*GP*GP*TP*CP*CP*TP*AP*GP*GP*AP*CP*CP*C)-3') | 18 | Homo sapiens | ![]() | ||
Sequence AnnotationsExpand | |||||
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Ligands 2 Unique | |||||
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ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
TTP (Subject of Investigation/LOI) Query on TTP | C [auth A] | THYMIDINE-5'-TRIPHOSPHATE C10 H17 N2 O14 P3 NHVNXKFIZYSCEB-XLPZGREQSA-N | |||
CA Query on CA | D [auth A], E [auth A] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |
Length ( Å ) | Angle ( ˚ ) |
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a = 97.556 | α = 90 |
b = 97.556 | β = 90 |
c = 202.148 | γ = 120 |
Software Name | Purpose |
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Coot | model building |
PHENIX | refinement |
XDS | data reduction |
XDS | data scaling |
PHENIX | phasing |
Funding Organization | Location | Grant Number |
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National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) | United States | GM081433 |